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KMID : 0616120120430030224
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Korean Journal of Pharmacognosy
2012 Volume.43 No. 3 p.224 ~ p.230
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Suppression of Th2-associated Cytokines Expression by Schizandrin in Basophils and Mast Cells
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Jeong Hwa-Hyun
Yoon Soo-Jeong
Pyo Myoung-Yun
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Abstract
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Schizandrin (SZ), was isolated from the fruit of Schizandra chinensis, has been reported to have many biological properties, including anti-inflammatory and antitumor activities. However, its anti-allergic effects are not completely elucidated. We focused on the anti-allergic effects of SZ in PMA/ionomycin (PI)-induced rat basophilic leukemia (RBL-2H3) cells and P815 mast cells. Cytokines (IL-4, IL-13), synthesized by basophils and mast cells, are implicated in pathological conditions such as asthma and allergy. The production of IL-4 and IL-13 was quantified by ELISA and the mRNA expression was detected by using RT-PCR assay. In this study, we found that SZ did not show cytotoxic effect at up to 100 on RBL-2H3 cells and mast cells. In addition, SZ inhibited the production of IL-4 and IL-13 and also decreased the level of mRNA in PI-induced RBL-2H3 cells and mast cells in a dose-dependent manner. Thus, we suggest that SZ may have the effect on preventing allergic disorders by inhibiting IL-4 and IL-13 cytokines.
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KEYWORD
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Schizandrin, Anti-allergy, Basophile, Mast cell, IL-4, IL-13
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